Efficient Enrichment of Bacterial mRNA from Host-Bacteria Total RNA Samples

Efficient Enrichment of Bacterial mRNA from Host-Bacteria Total RNA Samples

Despite numerous advances in genomics and bioinformatics, technological hurdles remain to examine host-microbe transcriptomics. Sometimes the transcriptome of either or both can be ascertained merely by generating more sequencing reads. However, many cases exist where bacterial mRNA needs to be enriched further to enable cost-effective sequencing of the pathogen or endosymbiont. While a suitabl...

Improved microRNA quantification in total RNA from clinical samples.

microRNAs are small regulatory RNAs that are currently emerging as new biomarkers for cancer and other diseases. In order for biomarkers to be useful in clinical settings, they should be accurately and reliably detected in clinical samples such as formalin fixed paraffin embedded (FFPE) sections and blood serum or plasma. These types of samples represent a challenge in terms of microRNA quantif...

Comparison of commercially-available RNA extraction methods for effective bacterial RNA isolation from milk spiked samples

Nucleic-acid based methods for bacterial identification are extremely useful in diagnostic applications due to their specificity and sensitivity. However, they require an optimal purification of the target molecules. As part of the development of a new diagnostic method for the detection of bacterial RNA in cow milk, we have compared four commercially available RNA extraction kits for the isola...

RNA isolation (Total RNA Extraction from High Polysaccharide Plants). Fatemeh Mehrpooyan*

General features of bacterial small RNA - mRNA interactions ?

In recent years small regulatory RNAs (sRNAs) in bacteria have received rapidly growing attention. The largest and most extensively studied group of sRNAs bind to trans-encoded target mRNAs to post-transcriptionally regulate their translation or alter their stability. Therefore, these sRNAs can be considered as functional analogs to eukaryotic microRNAs (miRNAs). Unlike miRNAs, sRNAs are hetero...